Journal: Drug Design, Development and Therapy

Article Title: MK-4 Ameliorates Diabetic Osteoporosis in Angiogenesis-Dependent Bone Formation by Promoting Mitophagy in Endothelial Cells

doi: 10.2147/DDDT.S503930

Figure Lengend Snippet: MK-4 ameliorates the impairments of type H vessel formation and angiogenesis-dependent bone formation in DOP mice. ( a ) Schematic drawing of the procedures conducted in this study. ( b and c ) Representative flow cytometry plots of CD31 hi Emcn hi ECs (type H ECs). The bar graph showed the quantitation of type H ECs. ( d and e ) Confocal image of CD31 hi and Emcn hi (type H blood vessels) in mice models and histomorphometric quantitation of the area of type H blood vessels. Scale bar: 50μm. ( f and g ) Confocal image of Osterix + (green) osteoprogenitors around CD31 hi vessels (red) and the quantitative analysis. Scale bar: 50μm. ( h ) Typical three-dimensional and two-dimensional coronal images of the tibias were obtained by Micro-CT. ( i ) HE and Masson staining of mice tibias. Scale bar: 200μm or 100μm. ( j ) Statistical analysis of bone histological parameters. ( k and l ) IHC analysis of ALP and RUNX2 in the tibias of mice models. Scale bar: 50μm. Data were presented as mean ± SD (n=5). * P < 0.05. ** P < 0.01. *** P < 0.001.

Article Snippet: The cells were subsequently washed and later incubated with PE-conjugated CD45 (E-AB-F1136D, 1:20; Elabscience, Wuhan, China) and APC-conjugated CD31 (E-AB-F1180E, 1:20; Elabscience) antibodies at 4 °C for 45 minutes.

Techniques: Flow Cytometry, Quantitation Assay, Micro-CT, Staining

Journal: Drug Design, Development and Therapy

Article Title: MK-4 Ameliorates Diabetic Osteoporosis in Angiogenesis-Dependent Bone Formation by Promoting Mitophagy in Endothelial Cells

doi: 10.2147/DDDT.S503930

Figure Lengend Snippet: 3-MA partially counteracts the promotion of type H vessel recovery and angiogenesis-dependent bone formation gain by MK-4. ( a and b ) Confocal image of CD31 hi and Emcn hi (termed type H blood vessels) in mice models and histomorphometric quantitation of the area of type H blood vessels. Scale bar: 50μm. ( c and d ) Confocal image of Osterix + (red) osteoprogenitors around Emcn hi vessels (green) and the quantitative analysis. Scale bar: 50μm. ( e ) HE and Masson staining of mice tibias. Scale bar: 200μm or 100μm. ( f ) Statistical analysis of bone histological parameters. ( g and h ) IHC analysis of ALP and RUNX2 in the tibias of mice models. Scale bar: 50μm. Data were presented as mean ± SD (n=5). * P < 0.05. ** P < 0.01. *** P < 0.001.

Article Snippet: The cells were subsequently washed and later incubated with PE-conjugated CD45 (E-AB-F1136D, 1:20; Elabscience, Wuhan, China) and APC-conjugated CD31 (E-AB-F1180E, 1:20; Elabscience) antibodies at 4 °C for 45 minutes.

Techniques: Quantitation Assay, Staining

Journal: Cell Reports Medicine

Article Title: A functional cardiac patch promotes cardiac repair by modulating the CCR2 − cardiac-resident macrophage niche and their cell crosstalk

doi: 10.1016/j.xcrm.2025.101932

Figure Lengend Snippet:

Article Snippet: PE Anti-Mouse CD31 Antibody , Elabscience , Cat# E-AB-F1180D; RRID: AB_3675229.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Staining, Labeling, H&E Stain, Lysis, Software

Journal: Journal of Cellular and Molecular Medicine

Article Title: Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model

doi: 10.1111/j.1582-4934.2010.01131.x

Figure Lengend Snippet: Sheep MSC were characterized using FACS and RT-PCR analysis. (A) With RT-PCR analysis CD29, CD44 and CD166 expression of MSC could be proofed on mRNA level. As indicated by increased CD45 expression, ratio of hematopoietic cells was higher in directly auto-transplanted MSC as compared to expanded MSC. (B–D) FACS analysis revealed sheep MSC to express CD29, CD44 and CD166. Expanded MSC (B) were negative for the hematopoietic markers CD31 and CD45. Directly auto-transplanted cells (C) had a different expression pattern than expanded MSC. The directly auto-transplanted MSC had a weaker CD29 and CD166 but a stronger CD45 expression. Mean fluorescent indices are shown in (D).

Article Snippet: CD31 staining: After antigen retrieval with pH 6 solution (Target Retrieval Solution; Dako Cytomation) in a pressure cooker for 10 min. (Pascal; Dako Cytomation) peroxidase block (CSAII-System; Dako Cytomation) was applied for 15 min., followed by incubation with 10% goat serum (PromoCell GmbH) in PBS (PBS-Dulbecco 1×, Biochrom AG) for 30 min. and protein block with the CSA II-System for 30 min. Then sections were incubated with the primary monoclonal mouse anti-ovine antibody CD31 (Anti-CD31/PECAM-1, MorphoSys UK Ltd., Kidlington, Oxford, UK) at 1:100 diluted in 10% goat serum in PBS for 1 hr.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model

doi: 10.1111/j.1582-4934.2010.01131.x

Figure Lengend Snippet: For determination of the cell type which is qualified best for bone tissue engineering purposes, different groups (expanded versus directly auto-transplanted MSC, groups 8–10) were investigated. In both groups cells were DiI labelled prior to implantation and implanted subcutaneously with or without BMP-2. (A–C) Expanded MSC (A), directly auto-transplanted MSC (B), BMP-2 in combination with directly auto-transplanted MSC (C). DiI-labelled MSC (red) could be found close to β-TCP/HA granules contributing to the newly formed bone parts. In the explants with directly auto-transplanted MSC a higher section of the DiI-labelled cells were found in the connective tissue parts of the constructs compared to the explants with expanded MSC or directly auto-transplanted MSC with BMP-2. (D–F) Sections of constructs of the groups with expanded MSC (D), directly auto-transplanted MSC (E), BMP-2 in combination with directly auto-transplanted MSC (F) were evaluated for vascularization. The constructs in all three groups are well vascularized as shown by CD31 immunohistochemistry (green). Nuclei are counterstained with DAPI (blue).

Article Snippet: CD31 staining: After antigen retrieval with pH 6 solution (Target Retrieval Solution; Dako Cytomation) in a pressure cooker for 10 min. (Pascal; Dako Cytomation) peroxidase block (CSAII-System; Dako Cytomation) was applied for 15 min., followed by incubation with 10% goat serum (PromoCell GmbH) in PBS (PBS-Dulbecco 1×, Biochrom AG) for 30 min. and protein block with the CSA II-System for 30 min. Then sections were incubated with the primary monoclonal mouse anti-ovine antibody CD31 (Anti-CD31/PECAM-1, MorphoSys UK Ltd., Kidlington, Oxford, UK) at 1:100 diluted in 10% goat serum in PBS for 1 hr.

Techniques: Construct, Immunohistochemistry

Journal: PLoS ONE

Article Title: Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Hepatic Ischemia Reperfusion Injury in a Rat Model

doi: 10.1371/journal.pone.0019195

Figure Lengend Snippet: A . Bright-field image. B–C . CD29 and CD105 surface antigens are positive. D . CD31 surfice antigen is negative, E . CD34 surfice antigen negative.

Article Snippet: The fluorescein (green, 1∶3000)-conjugated secondary antibody (COSMO BIO, Inc., Tokyo) against CD31, CD34, and CD105 was applied for 30 min. Rhodamine (red, 1∶3000)-conjugated secondary antibody (Rockland immunochemicals, Inc., Tokyo) against CD29 was also used.

Techniques: